Our Faculty

Marcy Kelly

Professor

Dyson College of Arts and Sciences

Biology

Location
  • @New York City
    One Pace Plaza W330

Education


BA ,
Biological Sciences

PhD
Microbiology and Molecular Genetics

Publications


Gulino, K. M., Zuzga, D. S. & Peteroy-Kelly, M. A. (2016, March). The Effects of M. bovis-BCG on the Vasodilator-Stimulated Phosphoprotein in the Macrophage Actin Filament Network. BIOS.

Brancaccio-Taras, L., Pape-Lindstrom, P., Peteroy-Kelly, M., Aguirre, K., Awong-Taylor, J., Balser, T., Cahill, M., Jack, T., Kelrick, M., Miller, K., Marley, K., Osgood, M., Romano, S. & Uzman, A. The PULSE Vision and Change Rubrics version 1.0; A Valid and Equitable Tool to Measure Life Sciences Department Transformation at all Institution Types. CBE: Life Sciences Education.

Pape-Lindstrom, P., Jack, T., Miller, K., Aguirre, K., Awong-Taylor, J., Balser, T., Loretta, B., Peteroy-Kelly, M., Marley, K., Osgood, M. & Romano, S. (2015, December). PULSE Pilot Certification Results. Journal of Microbiology and Biology Education. Vol 17 (Issue 2)

Kelly, M. A. (2015). The Fundamentals of Scientific Research: An Introductory Laboratory Manual..

Kelly, M. A. & Zuzga, D. (2013, August). Assessing a Year-Long, Research Lab in a Core Biology Course.. http://visionandchange.org/abstract/assessing-a-year-long-research-lab-in-a-core-biology-course/

PRESENTATIONS


Patel, N. D., Lawrence, R., Yancey, R. & Peteroy-Kelly, M. (2015, June). American Society for Microbiology General Meeting. Persistent Mycobacterium bovis-BCG is Resistant to Glutathione Induced Reductive Stress Killing.. American Society for Microbiology, New Orleans, LA

Buraei, Z., Marcello, M., Strahs, D., Zuzga, D., Crispo, E. & Peteroy-Kelly, M. (2015, May). American Society for Microbiology Conference for Undergraduate Educators 2015. Research-Based Biology in the Curriculum: Assessment of a Year-Long Cellular and Molecular Biology Laboratory. American Society for Microbiology, Austin, TX

Kelly, M. A., Strahs, D., Buraei, Z. K. & Marcello, M. R. (2015, May 30). ASMCUE 2015. Research-Based Biology in the Curriculum: Assessment of a Year-Long Cellular and Molecular Biology Laboratory. American Society for Microbiology, Austin, TX

Kelly, M. A. & Patel, N. D. (2014, June). American Society for Microbiology General Meeting. The Effect of Glutathione and its Derivatives on Mid-Logarithmic and Persistent Mycobacterium bovis-BCG.. American Society for Microbiology, Boston, MA

Kelly, M. A. (2014, April). Nyack College 3rd Annual Undergraduate Research Conference. Tuberculosis: A Multi-Disciplinary Response to a Global Threat.. Nyack College, Nyack, NY

RESEARCH INTEREST


1. Understanding the interaction between mycobacteria and small molecules produced by the human immune response. 2. Biology undergradauate edcuation reform.

Grants, Sponsored Research and Contracts

Kelly, M. (2016, January). Persistent Mycobacterium bovis-BCG is resistant to glutathione induced reductive stress killing..
Provost's Office , Pace University , $2,733.32 . Funded,

Kelly, M. A. (2015, October). Expanding Horizons in the Biology Community at Pace University-NYC.
Pace University, Dyson College, Mentoring Grant Funding , Pace University , $5,000.00 . Funded,

Kelly, M. A. (2014, June 2). Development of a Year-Long, Research-Based Laboratory Integrated within Core Genetics and Cellular and Molecular Biology Courses..
Americal Society for Microbiology Scholars Alumni Fellowship , Private , $800.00 . Funded,This fellowship was to purchase qualitative educational research software to analyze data from the NSF TUES funded study.

Kelly, M. A. (2014, January). Understanding the Impact of Glutathione on Persistant Mycobacterium bovis-BCG.
Pace University Scholarly Research , Pace University , $2,400.00 . Funded,Mycobacterium tuberculosis is responsible for nearly 2 million deaths yearly. Upon inhalation, mycobacteria are engulfed by alveolar macrophages. The immune defenses of these cells, including the reactive oxygen and nitrogen intermediates (ROI's and RNI's), are ineffective against invading mycobacteria. Formation of a tubercle to sequester the infected macrophages initiates latent tuberculosis infection, in which the mycobacteria enter a state of non-replicative persistence (NRP). Glutathione (GSH), a host tripeptide thiol-based detoxification molecule, protects host cells from ROI/RNI toxicity. We have demonstrated that the growth of mid-logarithmic M. bovis-BCG (BCG) is inhibited by 4 mM and 8 mM GSH. Fast growing mycobacteria displayed resistance to GSH. 8 mM GSH stimulates the growth of NRP BCG beginning at 4 days post-exposure by 4 fold compared to unexposed NRP BCG. Intracellular ATP concentrations in NRP BCG exposed to 8 mM GSH begin to rise 3 days after exposure to GSH. The NRP state seems to be protective with respect to GSH toxicity at a concentration of 8 mM. By day 4, 0.76% of mid-logarithmic cells exposed to 8 mM GSH were viable whereas 35% of NRP cells were viable. Nearly similar growth, percent viability, and intracellular ATP concentration results were found upon exposure of mid-logarithmic and NRP BCG to 8 mM GSNO. GSNO is a modified version of GSH that serves as a stable delivery system for the RNI, NO. HPLC analyses of the cytoplasms from unexposed and 8 mM GSH exposed mid-logarithmic and NRP BCG suggest that toxicity to GSH is not due to alterations in the redox potential in the mycobacterial cytoplasms. NRP BCG exposed to 8 mM GSH displayed a slight, but insignificant, decrease in mycothiol (MSH) levels at day 4 post-exposure but, the MSH levels returned to levels similar to that of unexposed NRP BCG the next day. MSH is the thiol detoxification molecule present in the cytoplasms of BCG. These data suggest that MSH may have a role in the growth stimulation and protective response to GSH seen in NRP BCG. The levels of all of the other thiols present in the mid-logarithmic and NRP BCG cytoplasms were the same under all conditions studied. Site-directed mutagenesis and transcriptional profiling studies are underway to tease out the molecular mechanisms responsible for these phenomena.

Kelly, M. A. (2014, October). Expanding Horizons in the Biology Community at Pace University-NYC.
Pace University, Dyson College, Mentoring Grant Funding , Pace University , $5,000.00 . Funded,

Buraei, Z. K., Kelly, M. A., Strahs, D. & Birney, L. (2013, July). Development of a Year-long, Research-based Laboratory Integrated within Core Genetics and Cellular & Molecular Biology Courses.
NSF , Federal , $180,272.00 . Funded,

Kelly, M. A. (2013, July 1). Development of a Year-Long, Reserach-Based Laboratory within Core Genetics and Cellular and Molecular Biology Courses..
National Science Foundation , Federal , $180,000.00 . Funded,This project expands and enriches a semester long research based molecular biology laboratory course into a novel year-long research based curriculum that emphasizes experimental design and execution, data interpretation and written and oral presentation skills as integral components of the course. The year-long approach has been piloted and preliminary assessment studies demonstrate significant learning gains by undergraduate students completing the two semester series. The year-long program will be completed by all biology majors and will be spread over two courses: Genetics (BIO231) and Introduction to Cellular and Molecular Biology (BIO335). In both courses, specific content areas covered in lab will predominately reflect the respective parent courses; during BIO231 students will develop and refine experimental methods to conduct and analyze a microarray experiment to study the effects of osmotic stress on yeast cells. These data will be used as a roadmap in the following semester in BIO335, where they will clone candidate genes involved in osmotic stress and ultimately, design and conduct cell-based functional assays to test hypotheses generated throughout the term. The project also pilots the addition of a service learning option, a science outreach program to serve high needs schools in New York City, as a feature of the course. INTELLECTUAL MERIT: Students in these revised courses employ an interdisciplinary approach, relying upon modern genomic, molecular and cell biology methodologies, including RNA-Seq technology, to answer a well-defined biological question. Interdisciplinary approaches are increasingly being emphasized in all STEM disciplines. In addition the writing component represents a trend that is beginning to emerge as an effective way to improve student learning gains. The results of the evaluation of this project should add to what is known about effective ways to improve undergraduate biology laboratories and for students and faculty to profit from introduction of research based student laboratories. Comparison of student scores on the Educational Testing Service Major Field Test in Biology are being used to assess effects on student learning, along with the use of surveys and focus groups, as well as the writing assignments to assess both learning and attitudinal changes. BROADER IMPACTS: Piloting of the service learning component adds to the broader impacts of this project. The project is participating in the CURE survey of Dr Lopatto, thus contributing to a national data base concerning the outcomes of research based student laboratories. Results are being widely disseminated through regional and national workshops and disciplinary annual meetings and publications.

Lawrence, R. (2014, June 1). The Persisstant State Protects Mycobacterium bovis-BCG from the Toxic Effects of Host Thiol-Based Detoxification Molecules..
American Society for Microbiology , Private , $5,000.00 . Funded,The research laboratory of Dr. Marcy Kelly focuses on elucidating the impact of the host immune system, thiol-based detoxification molecule, glutathione (GSH), on the survival of persistent mycobacteria. Thus far, work from Dr. Kelly's laboratory has confirmed the results of Green et al. (1) by demonstrating that the growth of mid-logarithmic M. bovis-BCG (BCG) was inhibited by 4 mM and 8 mM GSH. They also demonstrated that phenotypic persistence in BCG was not affected by exposure to 4 mM GSH. Specifically, the growth of the NRP mycobacteria exposed to 4 mM GSH was inhibited similar to that of unexposed NRP mycobacteria over a five day period. In contrast to these findings, the growth of NRP BCG was stimulated four fold following five days of exposure to 8 mM GSH. The NRP state seemed to be protective with respect to GSH toxicity at a concentration of 8 mM. By day 4, 0.76% of mid-logarithmic cells exposed to 8 mM GSH were viable whereas 35% of NRP cells were viable. Intracellular ATP concentrations in NRP BCG exposed to 8 mM GSH began to rise 3 days after exposure to GSH. In order to gain some insight into the molecular mechanisms responsible for the mycobacterial response to GSH, HPLC analyses of the thiol content of the cytoplasms from both mid-logarithmic and persistent BCG was performed. The HPLC data suggests that toxicity to 8 mM GSH in mid-logarithmic BCG was mostly likely due to alterations in the oxidative balance in the cytoplasms of the organisms. Mycobacteria have their own thiol-based detoxification system that utilizes mycothiol as the main mediator of cytoplasmic oxidative balance. HPLC analyses demonstrated that, by day five, there was a two-fold increase in the levels of a molecule with the same retention time as mycothiol in the cytoplasms of mid-logarithmic BCG exposed to 8 mM GSH. The increase in the levels of mycothiol suggests that the bacterium was attempting, unsuccessfully, to overcome the oxidative stress resulting from the presence of the GSH. In contrast to the findings with the mid-logarithmic BCG exposed to 8 mM GSH, the levels of mycothiol do not change significantly over the five day period in persistent BCG exposed to 8 mM GSH. These data confirm that the NRP state seems to protect BCG from the toxic effects of GSH because of the levels of mycothiol do not change in NRP BCG exposed to 8 mM GSH. The work proposed by Mr. Raheem Lawrence will enable the laboratory to continue to elucidate the mechanisms behind the response of BCG to GSH. Mr. Lawrence intends to use transposon mutagenesis to identify novel genes involved in the mycobacterial response to GSH. To these ends, we have already acquired the transposon system for this proposed study from the laboratory of Dr. Bill Jacobs from the Albert Einstein College of Medicine in NY. Additionally, we have been collaborating with Dr. Nancy Connell at Rutgers University in NJ. Preliminary, unpublished genetic transposon mutagenesis studies using M. tuberculosis H37Rv exposed to 4 mM GSH from Dr. Connell's laboratory demonstrated that resistance to GSH developed when several genes involved in fatty acid metabolism were deleted. Many of these same genes also play regulatory roles within mycobacterial cells such as fadD and mmpl (Connell, personal communication). It is anticipated that we will identify similar genes using BCG exposed to 8 mM GSH. Once we identify potential genes of interest, we will clone them into a mycobacterial single copy, shuttle vector and confirm their role in the response to GSH by transforming them into the deletion mutants and exposing them to GSH. Upon successfully performing the experiments proposed above, an understanding of the effect of GSH on BCG will enable us to develop an understanding about the interactions between latent mycobacteria and the human immune response. The overall goal from this work and the results produced will provide more information to aid in the development of vaccines to prevent the disease.

PROFESSIONAL MEMBERSHIPS

  • Partnership for Undergraduate Life Sciences Education [Fellow] , September 2012
    Organization developed in 2012 by major stakeholders in biology undergraduate education (NSF, NIH/NIGMS, HHMI, AAAS) to implement the core compencies and concepts outlined in the 2011 Vision and Change in Undergraduate Biology Education: A Call to Action report.
  • Beta Beta Beta , September 2000
    National undergraduate Biology honor society
  • American Society for Microbiology [Lead Facilitatory for Biology Scholars Program Transitions Residency; Lead Research Editor, Journal of Microbiology and Biology Education] , September 1999
    National, professional scientific society for individuals interested in microbiology

COLLEGE SERVICE

  • Peer Leader Training for Discussion Group Program [Faculty Mentor]
    Desc: Trained all BIO 101, CHE 111 and MAT discussion group peer leaders.

UNIVERSITY SERVICE

  • UNV 101 Advisory Board [Committee Chair]
    Desc: Tasked to perform a full assessment of UNV 101 and provide Provost with recommendations for the program
    Committee's Key Accomplishments: Our UNV 101 program is in line with national standards and best practices for first year experience courses.
  • Retention Committee [Committee Member]
    Desc: Attend Retention Committee meetings and provide suggestions. My work focused on providing committee information on the UNV 101 program and retention of STEM majors.
  • Announced Dyson Graduate Names [Attendee, Graduation]
    Desc: Announced names of Dyson graduates at the NYC undergraduate graduation ceremony.
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